There are 428 fewer teachers in Tayside and Fife’s secondary schools than a decade ago, official figures show.
In Dundee, the number of teachers at state-funded secondary schools has fallen by 24% since 2007 — the biggest drop in mainland Scotland.
The Tories said Dundee was suffering from the SNP’s blanket approach to teacher recruitment.
Education chiefs in the city said the closure of Menzieshill High School was among the reasons for the drop.
The number of teachers in Tayside and Fife fell from 3,791 in 2007 to 3,363 last year, according to Scottish Government figures.
The steepest drop in mainland Scotland was in the City of Discovery, where numbers fell from 805 to 608 over that period.
Bill Bowman, for the Scottish Conservatives, said the SNP was doing nothing to tackle the specific recruitment challenges in Dundee. He said: “Scotland once had an education system that was the envy of the world, and it has suffered blow after blow in the SNP’s hands. Dundee is one of the places suffering as a result.”
A spokesman for Dundee City Council said: “These latest figures reflect major changes within the school environment in the city, in particular the closure of Menzieshill High School and the increased activity being delivered through the attainment challenge.
“As with councils across the country, this has been a challenging time for teacher recruitment. The children and families service closely monitors teacher numbers in all the city’s schools and makes every effort to recruit candidates to fill these vacant posts.”
A spokeswoman for the Scottish Government said: “The fact is there are more secondary teachers in Scotland than at any time since 2014.
“What is more, the ratio of pupils to teachers is at its lowest since 2010 and, in Dundee, is well below the Scottish average.”