Irresponsible dog owners will be targeted in Newport and Tayport.
The Just Bin It campaign already used in other parts of the kingdom has been extended to the towns by Fife Council.
It comes after a number of locations in the area were identified as dog fouling hotspots.
Patrols by officers who can issue fines will be increased and signs, banners, pavement stencils and social media will be used to raise awareness of the consequences of people failing to pick up and dispose of dog mess.
People have been urged to report where and when they see dog fouling occur.